random extension plus kit (DuPont de Nemours)
90
Structured Review
DuPont de Nemours
random extension plus kit
Random Extension Plus Kit, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/random+extension+plus+kit/random+primer+extension+kit/pm16087725-80-50-54
Average 90 stars, based on 1 article reviews
Random Extension Plus Kit, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/random+extension+plus+kit/random+primer+extension+kit/pm16087725-80-50-54
Average 90 stars, based on 1 article reviews
random extension plus kit - by Bioz Stars,
2026-10
90/100 stars
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Plasmid Preparation:Article Title: Fish testicular 11beta-hydroxylase: cDNA cloning and mRNA expression during spermatogenesis. Article Snippet: Total RNA (10 ug) from each day was resolved by formaldehyde denaturing agarose gel electrophoresis, then transferred to a nylon membrane (Amersham). .. The full-lenzth cDNA of eel testis P450(110) was excised from vector; labeled with-[3ZP]dCTP using a Labeling:Article Title: Fish testicular 11beta-hydroxylase: cDNA cloning and mRNA expression during spermatogenesis. Article Snippet: Total RNA (10 ug) from each day was resolved by formaldehyde denaturing agarose gel electrophoresis, then transferred to a nylon membrane (Amersham). .. The full-lenzth cDNA of eel testis P450(110) was excised from vector; labeled with-[3ZP]dCTP using a Article Title: Isolation, characterization and expression of 11beta-hydroxysteroid dehydrogenase type 2 cDNAs from the testes of Japanese eel (Anguilla japonica) and Nile tilapia (Oreochromis niloticus). Article Snippet: .. This cDNA insert was then labeled by 32P-dCTP using the Article Title: Isolation, characterization and expression of 11beta-hydroxysteroid dehydrogenase type 2 cDNAs from the testes of Japanese eel (Anguilla japonica) and Nile tilapia (Oreochromis niloticus). Article Snippet: .. Ten micrograms total RNA from each sample were separated on a 1% formaldehyde-agarose denaturing gel, transferred to Hybond-N+ nylon membrane (Amersham) and baked at 80 C for 2 h. The full cDNA insert of eel 11 -HSD2 was labeled with 32P-dCTP using the Article Title: Cloning, expression and characterization of three types of 17beta-hydroxysteroid dehydrogenases from the Nile tilapia, Oreochromis niloticus. Article Snippet: Poly (A)+-RNAs were purified using Oligotex-dT30 (Takara, Otsu, Shiga, Japan). .. Then 5–8 μg mRNAs were applied to each well and were electrophoresed on a 1·5% (w/v) formaldehyde denatured agarose gel, transferred onto Hybond-N+ nylon membrane (Amersham, Little Chalfont, Buckinghamshire, England) and baked at 80 C for 2 h. The ORFs of three types of 17 -HSDs were labeled individually using cDNA Library Assay:Article Title: Isolation, characterization and expression of 11beta-hydroxysteroid dehydrogenase type 2 cDNAs from the testes of Japanese eel (Anguilla japonica) and Nile tilapia (Oreochromis niloticus). Article Snippet: .. This cDNA insert was then labeled by 32P-dCTP using the Membrane:Article Title: Isolation, characterization and expression of 11beta-hydroxysteroid dehydrogenase type 2 cDNAs from the testes of Japanese eel (Anguilla japonica) and Nile tilapia (Oreochromis niloticus). Article Snippet: .. Ten micrograms total RNA from each sample were separated on a 1% formaldehyde-agarose denaturing gel, transferred to Hybond-N+ nylon membrane (Amersham) and baked at 80 C for 2 h. The full cDNA insert of eel 11 -HSD2 was labeled with 32P-dCTP using the Article Title: Cloning, expression and characterization of three types of 17beta-hydroxysteroid dehydrogenases from the Nile tilapia, Oreochromis niloticus. Article Snippet: Poly (A)+-RNAs were purified using Oligotex-dT30 (Takara, Otsu, Shiga, Japan). .. Then 5–8 μg mRNAs were applied to each well and were electrophoresed on a 1·5% (w/v) formaldehyde denatured agarose gel, transferred onto Hybond-N+ nylon membrane (Amersham, Little Chalfont, Buckinghamshire, England) and baked at 80 C for 2 h. The ORFs of three types of 17 -HSDs were labeled individually using Amplification:Article Title: Ovarian mitochondrial cytochrome b mRNA levels increase with sexual maturity in freshwater eels (Anguilla spp.). Article Snippet: Differential display of mRNA was used to identify an upregulated gene in ovaries of artificially maturing Japanese eels (Anguilla japonica).. Accordingly, mitochondrial (mt) cytochrome b, whose transcript levels increased from early to late vitellogenesis, was isolated, cloned and sequenced.. Temporal trends in artificially maturing eels were compared with those in naturally and artificially maturing New Zealand eels (longfinned eel, Anguilla dieffenbachii; shortfinned eel, Anguilla australis) by Northern blot and in situ hybridization analysis to rule out any experimental artifacts. Agarose Gel Electrophoresis:Article Title: Cloning, expression and characterization of three types of 17beta-hydroxysteroid dehydrogenases from the Nile tilapia, Oreochromis niloticus. Article Snippet: Poly (A)+-RNAs were purified using Oligotex-dT30 (Takara, Otsu, Shiga, Japan). .. Then 5–8 μg mRNAs were applied to each well and were electrophoresed on a 1·5% (w/v) formaldehyde denatured agarose gel, transferred onto Hybond-N+ nylon membrane (Amersham, Little Chalfont, Buckinghamshire, England) and baked at 80 C for 2 h. The ORFs of three types of 17 -HSDs were labeled individually using |